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firefly luciferase reporter plasmid pkm53  (Addgene inc)


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    Structured Review

    Addgene inc firefly luciferase reporter plasmid pkm53
    Firefly Luciferase Reporter Plasmid Pkm53, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/luciferase+reporter+plasmid/pKM53+(Plasmid+%23154156)/pm41888882-90-12-17
    Average 94 stars, based on 1 article reviews
    firefly luciferase reporter plasmid pkm53 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Luciferase:

    Article Title: An intramuscular prime and mucosal boost vaccine regimen protects against lethal clade 2.3.4.4b H5N1 challenge in cynomolgus macaques.
    Article Snippet: Ninaad Lasrado, Liping Wang, Jinyan Liu, Annika Rössler, Jayeshbhai Chaudhari, Qixin Wang, Jonathon J.. Stone, Francisco Armando GranadosContreras, Jessica Wu, Dalia N. CabreraBarragan, Alejandra WallerPulido, Samuel J. Nangle, Krishna Shah, Reed Boduch, Siddhesh Warke, Anthony Cook, Christopher Kitajewski, Laurent Pessaint, Mark G. Lewis, Hanne Andersen, Amanda J. Martinot, Ryan P. McNamara, Dan H. Barouch*

    Article Title: Displayed and Encoded Antigens on Adenovirus Vectors Optimize Humoral and Cellular Immune Responses in Rhesus Macaques
    Article Snippet: .. Briefly, human embryonic kidney HEK293T cells (ATCC CRL_3216) were co-transfected with a luciferase reporter plasmid (pLenti-CMV Puro-Luc, Addgene), packaging construct psPAX2 (AIDS Resource and Reagent Program) and Spike protein expressing pcDNA3.1-SARS-CoV-2 SΔCT using lipofectamine 2000 (Thermo Fisher). ..

    Article Title: TGF-β2/OPTN/FOXC1/ miR-200 axis regulates actin dynamics in human trabecular meshwork cells
    Article Snippet: The 3′UTR FOXC1 was amplified by PCR from genomic DNA isolated from HEK293T cell lines and cloned into the pMIR-REPORT luciferase expression vector (Thermo Fisher Scientific). .. Luciferase reporter plasmid (100 ng) and pRL-TK control (200 ng for normalization; Addgene) were transfected with Dharmafect Duo (Dharmacon, GE Healthcare) transfection reagent into HEK293T cell lines seeded in 24-well plates (6 × 10 4 cells per well). .. For co-transfection experiments, 5 nM of synthetic miRNAs (pre-miRs, Qiagen) or 30 nM of miRNA inhibitors (Anti-miRs, Qiagen) were added to the above reactions.

    Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma.
    Article Snippet: .. HB cells were co-transfected with either the constructed luciferase reporter plasmid or Super 8x TOPFlash/FOPFlash (Addgene) and control Renilla luciferase vector. ..

    Article Title: hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis.
    Article Snippet: hMTR4 is an RNA helicase and an essential co-factor for the nuclear RNA exosome.. Its role in the p53 pathway and cell cycle control remains unknown.. Here, gainand loss-of-function analyses in cell models showed that hMTR4 could not affect p53 mRNA levels, but decreased the levels of p53 protein and its downstream target genes by promoting p53 ubiquitination and degradation, thus accelerating cell cycle progression.

    Article Title: The PERK/ATF4 pathway is required for metabolic reprogramming and progressive lung fibrosis
    Article Snippet: The pcDNA3.1 (Invitrogen), PERK WT (plasmid 21814), PERK DN (plasmid 36954), and IRE1α WT (plasmid 20744) vectors were purchased from Addgene. .. Ppargc1a gene expression was evaluated using a luciferase reporter plasmid from Bruce Spiegelman purchased from Addgene (plasmid 8887) ( ). .. Plasmids were transfected with X-tremeGene 9 Transfection Reagent (06365809001; Roche), according to the manufacturer’s protocol.

    Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma
    Article Snippet: .. HB cells were co-transfected with either the constructed luciferase reporter plasmid or Super 8x TOPFlash/FOPFlash (Addgene) and control Renilla luciferase vector. ..

    Article Title: The PERK/ATF4 pathway is required for metabolic reprogramming and progressive lung fibrosis.
    Article Snippet: The pcDNA3.1 (Invitrogen), PERKWT (plasmid 21814), PERKDN (plasmid 36954), and IRE1αWT (plasmid 20744) vectors were purchased from Addgene. .. Ppargc1a gene expression was evaluated using a luciferase reporter plasmid from Bruce Spiegelman purchased from Addgene (plasmid 8887) (61). .. Plasmids were transfected with X-tremeGene 9 Transfection Reagent (06365809001; Roche), according to the manufacturer’s protocol.

    Plasmid Preparation:

    Article Title: An intramuscular prime and mucosal boost vaccine regimen protects against lethal clade 2.3.4.4b H5N1 challenge in cynomolgus macaques.
    Article Snippet: Ninaad Lasrado, Liping Wang, Jinyan Liu, Annika Rössler, Jayeshbhai Chaudhari, Qixin Wang, Jonathon J.. Stone, Francisco Armando GranadosContreras, Jessica Wu, Dalia N. CabreraBarragan, Alejandra WallerPulido, Samuel J. Nangle, Krishna Shah, Reed Boduch, Siddhesh Warke, Anthony Cook, Christopher Kitajewski, Laurent Pessaint, Mark G. Lewis, Hanne Andersen, Amanda J. Martinot, Ryan P. McNamara, Dan H. Barouch*

    Article Title: Displayed and Encoded Antigens on Adenovirus Vectors Optimize Humoral and Cellular Immune Responses in Rhesus Macaques
    Article Snippet: .. Briefly, human embryonic kidney HEK293T cells (ATCC CRL_3216) were co-transfected with a luciferase reporter plasmid (pLenti-CMV Puro-Luc, Addgene), packaging construct psPAX2 (AIDS Resource and Reagent Program) and Spike protein expressing pcDNA3.1-SARS-CoV-2 SΔCT using lipofectamine 2000 (Thermo Fisher). ..

    Article Title: TGF-β2/OPTN/FOXC1/ miR-200 axis regulates actin dynamics in human trabecular meshwork cells
    Article Snippet: The 3′UTR FOXC1 was amplified by PCR from genomic DNA isolated from HEK293T cell lines and cloned into the pMIR-REPORT luciferase expression vector (Thermo Fisher Scientific). .. Luciferase reporter plasmid (100 ng) and pRL-TK control (200 ng for normalization; Addgene) were transfected with Dharmafect Duo (Dharmacon, GE Healthcare) transfection reagent into HEK293T cell lines seeded in 24-well plates (6 × 10 4 cells per well). .. For co-transfection experiments, 5 nM of synthetic miRNAs (pre-miRs, Qiagen) or 30 nM of miRNA inhibitors (Anti-miRs, Qiagen) were added to the above reactions.

    Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma.
    Article Snippet: .. HB cells were co-transfected with either the constructed luciferase reporter plasmid or Super 8x TOPFlash/FOPFlash (Addgene) and control Renilla luciferase vector. ..

    Article Title: hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis.
    Article Snippet: hMTR4 is an RNA helicase and an essential co-factor for the nuclear RNA exosome.. Its role in the p53 pathway and cell cycle control remains unknown.. Here, gainand loss-of-function analyses in cell models showed that hMTR4 could not affect p53 mRNA levels, but decreased the levels of p53 protein and its downstream target genes by promoting p53 ubiquitination and degradation, thus accelerating cell cycle progression.

    Article Title: The PERK/ATF4 pathway is required for metabolic reprogramming and progressive lung fibrosis
    Article Snippet: The pcDNA3.1 (Invitrogen), PERK WT (plasmid 21814), PERK DN (plasmid 36954), and IRE1α WT (plasmid 20744) vectors were purchased from Addgene. .. Ppargc1a gene expression was evaluated using a luciferase reporter plasmid from Bruce Spiegelman purchased from Addgene (plasmid 8887) ( ). .. Plasmids were transfected with X-tremeGene 9 Transfection Reagent (06365809001; Roche), according to the manufacturer’s protocol.

    Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma
    Article Snippet: .. HB cells were co-transfected with either the constructed luciferase reporter plasmid or Super 8x TOPFlash/FOPFlash (Addgene) and control Renilla luciferase vector. ..

    Article Title: The PERK/ATF4 pathway is required for metabolic reprogramming and progressive lung fibrosis.
    Article Snippet: The pcDNA3.1 (Invitrogen), PERKWT (plasmid 21814), PERKDN (plasmid 36954), and IRE1αWT (plasmid 20744) vectors were purchased from Addgene. .. Ppargc1a gene expression was evaluated using a luciferase reporter plasmid from Bruce Spiegelman purchased from Addgene (plasmid 8887) (61). .. Plasmids were transfected with X-tremeGene 9 Transfection Reagent (06365809001; Roche), according to the manufacturer’s protocol.

    Construct:

    Article Title: An intramuscular prime and mucosal boost vaccine regimen protects against lethal clade 2.3.4.4b H5N1 challenge in cynomolgus macaques.
    Article Snippet: Ninaad Lasrado, Liping Wang, Jinyan Liu, Annika Rössler, Jayeshbhai Chaudhari, Qixin Wang, Jonathon J.. Stone, Francisco Armando GranadosContreras, Jessica Wu, Dalia N. CabreraBarragan, Alejandra WallerPulido, Samuel J. Nangle, Krishna Shah, Reed Boduch, Siddhesh Warke, Anthony Cook, Christopher Kitajewski, Laurent Pessaint, Mark G. Lewis, Hanne Andersen, Amanda J. Martinot, Ryan P. McNamara, Dan H. Barouch*

    Article Title: Displayed and Encoded Antigens on Adenovirus Vectors Optimize Humoral and Cellular Immune Responses in Rhesus Macaques
    Article Snippet: .. Briefly, human embryonic kidney HEK293T cells (ATCC CRL_3216) were co-transfected with a luciferase reporter plasmid (pLenti-CMV Puro-Luc, Addgene), packaging construct psPAX2 (AIDS Resource and Reagent Program) and Spike protein expressing pcDNA3.1-SARS-CoV-2 SΔCT using lipofectamine 2000 (Thermo Fisher). ..

    Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma.
    Article Snippet: .. HB cells were co-transfected with either the constructed luciferase reporter plasmid or Super 8x TOPFlash/FOPFlash (Addgene) and control Renilla luciferase vector. ..

    Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma
    Article Snippet: .. HB cells were co-transfected with either the constructed luciferase reporter plasmid or Super 8x TOPFlash/FOPFlash (Addgene) and control Renilla luciferase vector. ..

    Expressing:

    Article Title: An intramuscular prime and mucosal boost vaccine regimen protects against lethal clade 2.3.4.4b H5N1 challenge in cynomolgus macaques.
    Article Snippet: Ninaad Lasrado, Liping Wang, Jinyan Liu, Annika Rössler, Jayeshbhai Chaudhari, Qixin Wang, Jonathon J.. Stone, Francisco Armando GranadosContreras, Jessica Wu, Dalia N. CabreraBarragan, Alejandra WallerPulido, Samuel J. Nangle, Krishna Shah, Reed Boduch, Siddhesh Warke, Anthony Cook, Christopher Kitajewski, Laurent Pessaint, Mark G. Lewis, Hanne Andersen, Amanda J. Martinot, Ryan P. McNamara, Dan H. Barouch*

    Article Title: Displayed and Encoded Antigens on Adenovirus Vectors Optimize Humoral and Cellular Immune Responses in Rhesus Macaques
    Article Snippet: .. Briefly, human embryonic kidney HEK293T cells (ATCC CRL_3216) were co-transfected with a luciferase reporter plasmid (pLenti-CMV Puro-Luc, Addgene), packaging construct psPAX2 (AIDS Resource and Reagent Program) and Spike protein expressing pcDNA3.1-SARS-CoV-2 SΔCT using lipofectamine 2000 (Thermo Fisher). ..

    Control:

    Article Title: TGF-β2/OPTN/FOXC1/ miR-200 axis regulates actin dynamics in human trabecular meshwork cells
    Article Snippet: The 3′UTR FOXC1 was amplified by PCR from genomic DNA isolated from HEK293T cell lines and cloned into the pMIR-REPORT luciferase expression vector (Thermo Fisher Scientific). .. Luciferase reporter plasmid (100 ng) and pRL-TK control (200 ng for normalization; Addgene) were transfected with Dharmafect Duo (Dharmacon, GE Healthcare) transfection reagent into HEK293T cell lines seeded in 24-well plates (6 × 10 4 cells per well). .. For co-transfection experiments, 5 nM of synthetic miRNAs (pre-miRs, Qiagen) or 30 nM of miRNA inhibitors (Anti-miRs, Qiagen) were added to the above reactions.

    Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma.
    Article Snippet: .. HB cells were co-transfected with either the constructed luciferase reporter plasmid or Super 8x TOPFlash/FOPFlash (Addgene) and control Renilla luciferase vector. ..

    Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma
    Article Snippet: .. HB cells were co-transfected with either the constructed luciferase reporter plasmid or Super 8x TOPFlash/FOPFlash (Addgene) and control Renilla luciferase vector. ..

    Transfection:

    Article Title: TGF-β2/OPTN/FOXC1/ miR-200 axis regulates actin dynamics in human trabecular meshwork cells
    Article Snippet: The 3′UTR FOXC1 was amplified by PCR from genomic DNA isolated from HEK293T cell lines and cloned into the pMIR-REPORT luciferase expression vector (Thermo Fisher Scientific). .. Luciferase reporter plasmid (100 ng) and pRL-TK control (200 ng for normalization; Addgene) were transfected with Dharmafect Duo (Dharmacon, GE Healthcare) transfection reagent into HEK293T cell lines seeded in 24-well plates (6 × 10 4 cells per well). .. For co-transfection experiments, 5 nM of synthetic miRNAs (pre-miRs, Qiagen) or 30 nM of miRNA inhibitors (Anti-miRs, Qiagen) were added to the above reactions.

    Article Title: hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis.
    Article Snippet: hMTR4 is an RNA helicase and an essential co-factor for the nuclear RNA exosome.. Its role in the p53 pathway and cell cycle control remains unknown.. Here, gainand loss-of-function analyses in cell models showed that hMTR4 could not affect p53 mRNA levels, but decreased the levels of p53 protein and its downstream target genes by promoting p53 ubiquitination and degradation, thus accelerating cell cycle progression.

    Activity Assay:

    Article Title: hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis.
    Article Snippet: hMTR4 is an RNA helicase and an essential co-factor for the nuclear RNA exosome.. Its role in the p53 pathway and cell cycle control remains unknown.. Here, gainand loss-of-function analyses in cell models showed that hMTR4 could not affect p53 mRNA levels, but decreased the levels of p53 protein and its downstream target genes by promoting p53 ubiquitination and degradation, thus accelerating cell cycle progression.

    Mutagenesis:

    Article Title: hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis.
    Article Snippet: hMTR4 is an RNA helicase and an essential co-factor for the nuclear RNA exosome.. Its role in the p53 pathway and cell cycle control remains unknown.. Here, gainand loss-of-function analyses in cell models showed that hMTR4 could not affect p53 mRNA levels, but decreased the levels of p53 protein and its downstream target genes by promoting p53 ubiquitination and degradation, thus accelerating cell cycle progression.

    Binding Assay:

    Article Title: hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis.
    Article Snippet: hMTR4 is an RNA helicase and an essential co-factor for the nuclear RNA exosome.. Its role in the p53 pathway and cell cycle control remains unknown.. Here, gainand loss-of-function analyses in cell models showed that hMTR4 could not affect p53 mRNA levels, but decreased the levels of p53 protein and its downstream target genes by promoting p53 ubiquitination and degradation, thus accelerating cell cycle progression.

    Gene Expression:

    Article Title: The PERK/ATF4 pathway is required for metabolic reprogramming and progressive lung fibrosis
    Article Snippet: The pcDNA3.1 (Invitrogen), PERK WT (plasmid 21814), PERK DN (plasmid 36954), and IRE1α WT (plasmid 20744) vectors were purchased from Addgene. .. Ppargc1a gene expression was evaluated using a luciferase reporter plasmid from Bruce Spiegelman purchased from Addgene (plasmid 8887) ( ). .. Plasmids were transfected with X-tremeGene 9 Transfection Reagent (06365809001; Roche), according to the manufacturer’s protocol.

    Article Title: The PERK/ATF4 pathway is required for metabolic reprogramming and progressive lung fibrosis.
    Article Snippet: The pcDNA3.1 (Invitrogen), PERKWT (plasmid 21814), PERKDN (plasmid 36954), and IRE1αWT (plasmid 20744) vectors were purchased from Addgene. .. Ppargc1a gene expression was evaluated using a luciferase reporter plasmid from Bruce Spiegelman purchased from Addgene (plasmid 8887) (61). .. Plasmids were transfected with X-tremeGene 9 Transfection Reagent (06365809001; Roche), according to the manufacturer’s protocol.



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    86
    Genechem luciferase reporter plasmids
    ABLIM1 is a downstream target of miR-378a-5p and involves in abdominal aortic aneurysm development. (A) Volcano plot of differently expressed genes in GSE183464 and GSE237229 database. Venn diagram showed intersection of differentially expressed genes and predicted target genes including ABLIM1 , DDX5 and SLC7A1 . (B) RT-qPCR analysis of target genes Ablim1 , Ddx5 and Slc7a1 in the TNFα-treated vascular smooth muscle cells (n=3 per group). RT-qPCR analysis of Ablim1 , Ddx5 and Slc7a1 in the aortas of Ang II-treated mice (n=3 per group). (C) ABLIM1 expression in the aortas treated-with antagomir-NC or antagomir-378a-5p was identified using by immunofluorescence staining. ABLIM1 (red), α-SMA (green) and DAPI (blue). (D) Representative images of immunofluorescence staining identified ABLIM1 expression in aortas with angomir-NC or angomir-378a-5p. ABLIM1 (red), α-SMA (green) and DAPI (blue). (E) Conservatism analysis of the binding site for miR-378a-5p and ABLIM1 in in humans, mice and rat. (F) Luciferase activity in 293T cells transfected with mimics-miR-378a-5p together with ABLIM1 <t>-3'UTR-wild</t> type or mutant plasmid. Data are presented as the mean ± SEM. P-values were calculated by Student's t test (for B). ** P<0.01 vs. Control or saline or mimics-NC- ABLIM1 -3'UTR-WT. ABLIM1, actin-binding LIM protein 1; miR or miRNA, microRNA; RT-qPCR, reverse transcription-quantitative PCR; α-SMA, α-smooth muscle actin; NC, negative control; UTR, untranslated region; WT, wild-type; MUT, mutated.
    Luciferase Reporter Plasmids, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ABLIM1 is a downstream target of miR-378a-5p and involves in abdominal aortic aneurysm development. (A) Volcano plot of differently expressed genes in GSE183464 and GSE237229 database. Venn diagram showed intersection of differentially expressed genes and predicted target genes including ABLIM1 , DDX5 and SLC7A1 . (B) RT-qPCR analysis of target genes Ablim1 , Ddx5 and Slc7a1 in the TNFα-treated vascular smooth muscle cells (n=3 per group). RT-qPCR analysis of Ablim1 , Ddx5 and Slc7a1 in the aortas of Ang II-treated mice (n=3 per group). (C) ABLIM1 expression in the aortas treated-with antagomir-NC or antagomir-378a-5p was identified using by immunofluorescence staining. ABLIM1 (red), α-SMA (green) and DAPI (blue). (D) Representative images of immunofluorescence staining identified ABLIM1 expression in aortas with angomir-NC or angomir-378a-5p. ABLIM1 (red), α-SMA (green) and DAPI (blue). (E) Conservatism analysis of the binding site for miR-378a-5p and ABLIM1 in in humans, mice and rat. (F) Luciferase activity in 293T cells transfected with mimics-miR-378a-5p together with ABLIM1 -3'UTR-wild type or mutant plasmid. Data are presented as the mean ± SEM. P-values were calculated by Student's t test (for B). ** P<0.01 vs. Control or saline or mimics-NC- ABLIM1 -3'UTR-WT. ABLIM1, actin-binding LIM protein 1; miR or miRNA, microRNA; RT-qPCR, reverse transcription-quantitative PCR; α-SMA, α-smooth muscle actin; NC, negative control; UTR, untranslated region; WT, wild-type; MUT, mutated.

    Journal: International Journal of Molecular Medicine

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways

    doi: 10.3892/ijmm.2026.5768

    Figure Lengend Snippet: ABLIM1 is a downstream target of miR-378a-5p and involves in abdominal aortic aneurysm development. (A) Volcano plot of differently expressed genes in GSE183464 and GSE237229 database. Venn diagram showed intersection of differentially expressed genes and predicted target genes including ABLIM1 , DDX5 and SLC7A1 . (B) RT-qPCR analysis of target genes Ablim1 , Ddx5 and Slc7a1 in the TNFα-treated vascular smooth muscle cells (n=3 per group). RT-qPCR analysis of Ablim1 , Ddx5 and Slc7a1 in the aortas of Ang II-treated mice (n=3 per group). (C) ABLIM1 expression in the aortas treated-with antagomir-NC or antagomir-378a-5p was identified using by immunofluorescence staining. ABLIM1 (red), α-SMA (green) and DAPI (blue). (D) Representative images of immunofluorescence staining identified ABLIM1 expression in aortas with angomir-NC or angomir-378a-5p. ABLIM1 (red), α-SMA (green) and DAPI (blue). (E) Conservatism analysis of the binding site for miR-378a-5p and ABLIM1 in in humans, mice and rat. (F) Luciferase activity in 293T cells transfected with mimics-miR-378a-5p together with ABLIM1 -3'UTR-wild type or mutant plasmid. Data are presented as the mean ± SEM. P-values were calculated by Student's t test (for B). ** P<0.01 vs. Control or saline or mimics-NC- ABLIM1 -3'UTR-WT. ABLIM1, actin-binding LIM protein 1; miR or miRNA, microRNA; RT-qPCR, reverse transcription-quantitative PCR; α-SMA, α-smooth muscle actin; NC, negative control; UTR, untranslated region; WT, wild-type; MUT, mutated.

    Article Snippet: The 3' untranslated region (3'UTR) of mouse Ablim1 , Ddx5 (Dead-box helicase 5), Slc7a1 (Cationic amino acid transporter 1) gene was amplified and cloned into the pMIR-REPORT Luciferase (OBiO Technology Company) to construct pMIR-REPORT Luciferase 3'UTR wild-type (WT) plasmids.

    Techniques: Quantitative RT-PCR, Expressing, Immunofluorescence, Staining, Binding Assay, Luciferase, Activity Assay, Transfection, Mutagenesis, Plasmid Preparation, Control, Saline, Reverse Transcription, Real-time Polymerase Chain Reaction, Negative Control