firefly luciferase reporter plasmid pkm53 (Addgene inc)
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Firefly Luciferase Reporter Plasmid Pkm53, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luciferase+reporter+plasmid/pKM53+(Plasmid+%23154156)/pm41888882-90-12-17
Average 94 stars, based on 1 article reviews
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Luciferase:Article Title: An intramuscular prime and mucosal boost vaccine regimen protects against lethal clade 2.3.4.4b H5N1 challenge in cynomolgus macaques. Article Snippet: Ninaad Lasrado, Liping Wang, Jinyan Liu, Annika Rössler, Jayeshbhai Chaudhari, Qixin Wang, Jonathon J.. Stone, Francisco Armando GranadosContreras, Jessica Wu, Dalia N. CabreraBarragan, Alejandra WallerPulido, Samuel J. Nangle, Krishna Shah, Reed Boduch, Siddhesh Warke, Anthony Cook, Christopher Kitajewski, Laurent Pessaint, Mark G. Lewis, Hanne Andersen, Amanda J. Martinot, Ryan P. McNamara, Dan H. Barouch* Article Title: Displayed and Encoded Antigens on Adenovirus Vectors Optimize Humoral and Cellular Immune Responses in Rhesus Macaques Article Snippet: .. Briefly, human embryonic kidney HEK293T cells (ATCC CRL_3216) were co-transfected with a Article Title: TGF-β2/OPTN/FOXC1/ miR-200 axis regulates actin dynamics in human trabecular meshwork cells Article Snippet: The 3′UTR FOXC1 was amplified by PCR from genomic DNA isolated from HEK293T cell lines and cloned into the pMIR-REPORT luciferase expression vector (Thermo Fisher Scientific). .. Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma. Article Snippet: .. HB cells were co-transfected with either the constructed Article Title: hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis. Article Snippet: hMTR4 is an RNA helicase and an essential co-factor for the nuclear RNA exosome.. Its role in the p53 pathway and cell cycle control remains unknown.. Here, gainand loss-of-function analyses in cell models showed that hMTR4 could not affect p53 mRNA levels, but decreased the levels of p53 protein and its downstream target genes by promoting p53 ubiquitination and degradation, thus accelerating cell cycle progression. Article Title: The PERK/ATF4 pathway is required for metabolic reprogramming and progressive lung fibrosis Article Snippet: The pcDNA3.1 (Invitrogen), PERK WT (plasmid 21814), PERK DN (plasmid 36954), and IRE1α WT (plasmid 20744) vectors were purchased from Addgene. .. Ppargc1a gene expression was evaluated using a Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma Article Snippet: .. HB cells were co-transfected with either the constructed Article Title: The PERK/ATF4 pathway is required for metabolic reprogramming and progressive lung fibrosis. Article Snippet: The pcDNA3.1 (Invitrogen), PERKWT (plasmid 21814), PERKDN (plasmid 36954), and IRE1αWT (plasmid 20744) vectors were purchased from Addgene. .. Ppargc1a gene expression was evaluated using a Plasmid Preparation:Article Title: An intramuscular prime and mucosal boost vaccine regimen protects against lethal clade 2.3.4.4b H5N1 challenge in cynomolgus macaques. Article Snippet: Ninaad Lasrado, Liping Wang, Jinyan Liu, Annika Rössler, Jayeshbhai Chaudhari, Qixin Wang, Jonathon J.. Stone, Francisco Armando GranadosContreras, Jessica Wu, Dalia N. CabreraBarragan, Alejandra WallerPulido, Samuel J. Nangle, Krishna Shah, Reed Boduch, Siddhesh Warke, Anthony Cook, Christopher Kitajewski, Laurent Pessaint, Mark G. Lewis, Hanne Andersen, Amanda J. Martinot, Ryan P. McNamara, Dan H. Barouch* Article Title: Displayed and Encoded Antigens on Adenovirus Vectors Optimize Humoral and Cellular Immune Responses in Rhesus Macaques Article Snippet: .. Briefly, human embryonic kidney HEK293T cells (ATCC CRL_3216) were co-transfected with a Article Title: TGF-β2/OPTN/FOXC1/ miR-200 axis regulates actin dynamics in human trabecular meshwork cells Article Snippet: The 3′UTR FOXC1 was amplified by PCR from genomic DNA isolated from HEK293T cell lines and cloned into the pMIR-REPORT luciferase expression vector (Thermo Fisher Scientific). .. Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma. Article Snippet: .. HB cells were co-transfected with either the constructed Article Title: hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis. Article Snippet: hMTR4 is an RNA helicase and an essential co-factor for the nuclear RNA exosome.. Its role in the p53 pathway and cell cycle control remains unknown.. Here, gainand loss-of-function analyses in cell models showed that hMTR4 could not affect p53 mRNA levels, but decreased the levels of p53 protein and its downstream target genes by promoting p53 ubiquitination and degradation, thus accelerating cell cycle progression. Article Title: The PERK/ATF4 pathway is required for metabolic reprogramming and progressive lung fibrosis Article Snippet: The pcDNA3.1 (Invitrogen), PERK WT (plasmid 21814), PERK DN (plasmid 36954), and IRE1α WT (plasmid 20744) vectors were purchased from Addgene. .. Ppargc1a gene expression was evaluated using a Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma Article Snippet: .. HB cells were co-transfected with either the constructed Article Title: The PERK/ATF4 pathway is required for metabolic reprogramming and progressive lung fibrosis. Article Snippet: The pcDNA3.1 (Invitrogen), PERKWT (plasmid 21814), PERKDN (plasmid 36954), and IRE1αWT (plasmid 20744) vectors were purchased from Addgene. .. Ppargc1a gene expression was evaluated using a Construct:Article Title: An intramuscular prime and mucosal boost vaccine regimen protects against lethal clade 2.3.4.4b H5N1 challenge in cynomolgus macaques. Article Snippet: Ninaad Lasrado, Liping Wang, Jinyan Liu, Annika Rössler, Jayeshbhai Chaudhari, Qixin Wang, Jonathon J.. Stone, Francisco Armando GranadosContreras, Jessica Wu, Dalia N. CabreraBarragan, Alejandra WallerPulido, Samuel J. Nangle, Krishna Shah, Reed Boduch, Siddhesh Warke, Anthony Cook, Christopher Kitajewski, Laurent Pessaint, Mark G. Lewis, Hanne Andersen, Amanda J. Martinot, Ryan P. McNamara, Dan H. Barouch* Article Title: Displayed and Encoded Antigens on Adenovirus Vectors Optimize Humoral and Cellular Immune Responses in Rhesus Macaques Article Snippet: .. Briefly, human embryonic kidney HEK293T cells (ATCC CRL_3216) were co-transfected with a Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma. Article Snippet: .. HB cells were co-transfected with either the constructed Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma Article Snippet: .. HB cells were co-transfected with either the constructed Expressing:Article Title: An intramuscular prime and mucosal boost vaccine regimen protects against lethal clade 2.3.4.4b H5N1 challenge in cynomolgus macaques. Article Snippet: Ninaad Lasrado, Liping Wang, Jinyan Liu, Annika Rössler, Jayeshbhai Chaudhari, Qixin Wang, Jonathon J.. Stone, Francisco Armando GranadosContreras, Jessica Wu, Dalia N. CabreraBarragan, Alejandra WallerPulido, Samuel J. Nangle, Krishna Shah, Reed Boduch, Siddhesh Warke, Anthony Cook, Christopher Kitajewski, Laurent Pessaint, Mark G. Lewis, Hanne Andersen, Amanda J. Martinot, Ryan P. McNamara, Dan H. Barouch* Article Title: Displayed and Encoded Antigens on Adenovirus Vectors Optimize Humoral and Cellular Immune Responses in Rhesus Macaques Article Snippet: .. Briefly, human embryonic kidney HEK293T cells (ATCC CRL_3216) were co-transfected with a Control:Article Title: TGF-β2/OPTN/FOXC1/ miR-200 axis regulates actin dynamics in human trabecular meshwork cells Article Snippet: The 3′UTR FOXC1 was amplified by PCR from genomic DNA isolated from HEK293T cell lines and cloned into the pMIR-REPORT luciferase expression vector (Thermo Fisher Scientific). .. Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma. Article Snippet: .. HB cells were co-transfected with either the constructed Article Title: Therapeutic targeting de novo purine biosynthesis driven by β-catenin-dependent PPAT upregulation in hepatoblastoma Article Snippet: .. HB cells were co-transfected with either the constructed Transfection:Article Title: TGF-β2/OPTN/FOXC1/ miR-200 axis regulates actin dynamics in human trabecular meshwork cells Article Snippet: The 3′UTR FOXC1 was amplified by PCR from genomic DNA isolated from HEK293T cell lines and cloned into the pMIR-REPORT luciferase expression vector (Thermo Fisher Scientific). .. Article Title: hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis. Article Snippet: hMTR4 is an RNA helicase and an essential co-factor for the nuclear RNA exosome.. Its role in the p53 pathway and cell cycle control remains unknown.. Here, gainand loss-of-function analyses in cell models showed that hMTR4 could not affect p53 mRNA levels, but decreased the levels of p53 protein and its downstream target genes by promoting p53 ubiquitination and degradation, thus accelerating cell cycle progression. Activity Assay:Article Title: hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis. Article Snippet: hMTR4 is an RNA helicase and an essential co-factor for the nuclear RNA exosome.. Its role in the p53 pathway and cell cycle control remains unknown.. Here, gainand loss-of-function analyses in cell models showed that hMTR4 could not affect p53 mRNA levels, but decreased the levels of p53 protein and its downstream target genes by promoting p53 ubiquitination and degradation, thus accelerating cell cycle progression. Mutagenesis:Article Title: hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis. Article Snippet: hMTR4 is an RNA helicase and an essential co-factor for the nuclear RNA exosome.. Its role in the p53 pathway and cell cycle control remains unknown.. Here, gainand loss-of-function analyses in cell models showed that hMTR4 could not affect p53 mRNA levels, but decreased the levels of p53 protein and its downstream target genes by promoting p53 ubiquitination and degradation, thus accelerating cell cycle progression. Binding Assay:Article Title: hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis. Article Snippet: hMTR4 is an RNA helicase and an essential co-factor for the nuclear RNA exosome.. Its role in the p53 pathway and cell cycle control remains unknown.. Here, gainand loss-of-function analyses in cell models showed that hMTR4 could not affect p53 mRNA levels, but decreased the levels of p53 protein and its downstream target genes by promoting p53 ubiquitination and degradation, thus accelerating cell cycle progression. Gene Expression:Article Title: The PERK/ATF4 pathway is required for metabolic reprogramming and progressive lung fibrosis Article Snippet: The pcDNA3.1 (Invitrogen), PERK WT (plasmid 21814), PERK DN (plasmid 36954), and IRE1α WT (plasmid 20744) vectors were purchased from Addgene. .. Ppargc1a gene expression was evaluated using a Article Title: The PERK/ATF4 pathway is required for metabolic reprogramming and progressive lung fibrosis. Article Snippet: The pcDNA3.1 (Invitrogen), PERKWT (plasmid 21814), PERKDN (plasmid 36954), and IRE1αWT (plasmid 20744) vectors were purchased from Addgene. .. Ppargc1a gene expression was evaluated using a |
